Analyzing Biomolecular Interactions by Mass Spectrometry by Jeroen Kool, Wilfried M. A. Niessen

By Jeroen Kool, Wilfried M. A. Niessen

This monograph stories all correct applied sciences in line with mass spectrometry which are used to check or monitor organic interactions ordinarily.
prepared in 3 components, the textual content starts by way of reviewing recommendations these days nearly thought of classical, resembling affinity chromatography and ultrafiltration, in addition to the most recent thoughts. the second one half focusses on all MS-based tools for the examine of interactions of proteins with all periods of biomolecules. in addition to pull down-based ways, this part additionally emphasizes using ion mobility MS, capture-compound techniques, chemical proteomics and interactomics. The 3rd and ultimate half discusses different vital applied sciences usually hired in interplay reports, akin to biosensors and microarrays.
For pharmaceutical, analytical, protein, environmental and biochemists, in addition to these operating in pharmaceutical and analytical laboratories.

Show description

Read Online or Download Analyzing Biomolecular Interactions by Mass Spectrometry PDF

Best analytic books

Analytic Number Theory and Diophantine Problems: Proceedings of a Conference at Oklahoma State University, 1984

A convention on Analytic quantity concept and Diophantine difficulties was once held from June 24 to July three, 1984 on the Oklahoma country collage in Stillwater. The convention was once funded by means of the nationwide technological know-how beginning, the school of Arts and Sciences and the dep. of arithmetic at Oklahoma kingdom collage.

Practical Laboratory Automation: Made Easy with AutoIt

Via final the space among normal programming books and people on laboratory automation, this well timed booklet makes available to each laboratory technician or scientist what has routinely been constrained to hugely really good pros. Following the assumption of "learning through doing", the e-book presents an advent to scripting utilizing AutoIt, with many practicable examples in line with real-world eventualities.

Additional info for Analyzing Biomolecular Interactions by Mass Spectrometry

Example text

The C-trap fragmentation resembles the collision-cell CID more than it resembles the ion-trap–CID. Subsequently, separate higher-energy RF-only collision octapoles (higher-energy collision-induced dissociation, HCD) were mounted on LIT–orbitrap hybrid systems to make optimum use of this feature. Such a system can be considered a gas-phase ion-chemistry laboratory on its own, featuring different ways to perform fragmentation, that is, ion-trap CID, HCD, and eventually ETD, as well as different ways to measure the m/z values of the resulting ions, that is, by unit-mass resolution with the ion trap or by ultra-high resolution with the orbitrap.

Ions with mass m and z elementary charges e are accelerated with a voltage V into a magnetic field B with a path with a radius of curvature r. One can derive the equation m∕z = B2 r2 e∕2V , which indicates that the separation of ions with different m/z can be achieved in three different ways: by variation of the radius of curvature ions with different m/z are separated in space, while by variation of either B or V ions of different m/z are separated in time, that is, they can be detected one after another by a single-point detector at a fixed position behind a slit [44].

Whereas the conventional drift-tube approach has been the first way to perform ion mobility in combination with MS, drift-tube IMS–MS systems have become commercially available only very recently (2014).

Download PDF sample

Rated 4.11 of 5 – based on 13 votes